Tempeh extract fortified with iron and synbiotic as a strategy against anemia
2016
Kandarina, I. | Sudargo, T. | Wisnusanti, S. U. | Puspitaningrum, V. A. D. | Helmyati, S. | Juffrie, M. | Yuliati, E.
Iron fortification is one of potential way to overcome anemia. Probiotics can maintain digestive tract health while prebiotics can improve the absorption of minerals. Tempeh is rich in nutrientsrequired for hemoglobin synthesis, such as protein, vitamin B12, vitamin C, zinc, iron, and copper. The objective of this study was to evaluate the efficacy of tempeh extract fortified with iron and synbiotic in increasing the hemoglobin concentration (Hb). Fermented synbiotic tempeh extract was made using Lactobacillus plantarum Dad13 and fructo-oligosaccharides. A total of 24 male and female Wistar rats underwent the iron depletion phase to induce anemia, then divided into 3 groups, given: 1) Fermented synbiotic tempeh extract with 50 ppm Fe/ FeSO4 (Fe), 2) Fermented synbiotic tempeh extract (St), and 3) not receive any interventions (Co). The rats were fed with interventional diet AIN-93 free Fe during intervention. Body weight and Hb were measured before and after intervention for 17 days. There was a significant increase in body weight on Fe and St groups after intervention respectively, 31 g and 32 g, as well as for Hb, from 6.41 ± 0.14 to 11.48 ± 0.31 and 6.47 ± 0.23 to 11.03 ± 0.35 mg/dl (p <0.05). Group without intervention (Co) did not show significant increase both in body weight and hemoglobin. This can be a good opportunity to combat anemia using Indonesian local food, tempeh which fortified with iron and synbiotic.
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