Evaluation on the cultural characteristics and antagonistic activities of Cladobotryum semicirculare against Ganoderma boninense in vitro
2015
Goh, You Keng | Goh, Yit Kheng | Goh, Kah Joo | Nurul Fadhilah Marzuki | Tung, Hun Jiat
The objectives of this preliminary study were to identify the Cladobotryum-like isolate and assess the antagonistic activity of this fungus against G. boninense and other fungal pathogens. Based on both morphological and molecular approaches, the unknown fungus was later described as Cladobotryum semicirculare. Under dual-culture assays, Cladobotryum semicirculare was observed to suppress radial mycelial growth of all the tested fungi with G. lucidum and G. boninense G37’s growth beinginhibited the most, 74.8% and 74.7%, respectively. Clonostachys sp. inhibited the least (12.7%). Through poison agar test, filtrates from C. semicirculare at 50% concentration were observed to suppress the mycelial growth of G. boninense G14 (35% inhibition) and G. lucidum (25% suppression), and with 100% filtrate concentration both Ganoderma species were showing more than 49% inhibition. Growthof all tested Ganoderma isolates and C. semicirculare, was faster under aerobic (2.2 to 3.8 mm per day for Ganoderma isolates and 4.3 mm for Cladobotryum) compared to anaerobic (1.6 to 2.2 mm per day for Ganoderma isolates and 1.4 mm per day for Cladobotryum) condition. In vitro mycoparasitism test confirmed that C. semicirculare reduced the re-isolation of G. lucidum (33.3%) and G. boninense (66.7%) mycelia. This study also provided the first report of G. boninense as the host for C. semicirculare.
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