Isolation of A cDNA encoding cytosolic phosphoglucomutase from ripening chilli fruit
2000
Wizana Zainal Abidin | Zainon Mohd Ali | Zamri Zainal
Phosphoglucomutase (PGM, EC 5.4.2.2), which catalyzes the interconversion of glucose 1- and glucose 6-phosphate, is one of the enzymes that may be involved in the maintenance of equilibrium concentrations of relevant sugars at the hexose monophosphate (HMP) junction. This HMP junction, which include also the interconversion of fructose-6-phosphate and glucose-6-phosphate, represents important points in the metabolic pathways of cellular carbohydrates and other cellular components. We have isolated and partially sequenced a cDNA clone encoding cytosolic phosphoglucomutase from the library of ripening chilli (Capsicum annuum L. cv MCII), and designated capPGMl. This cDNA clone shows high homology at the deduced amino acid level with the cytosolic form of PGM from Solanum tuberosum (95%), Arabidopsis thaliana (83%), Pisum sativum (84%) and cambium of poplar (Populus tremula L. x P. tremuloides L.) (83%). Northern blot analysis seems to indicate that capPGMl was expressed throughout ripening.
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