Biochemical analyses of seeds of selected rice cultivars
2003
Yap W.S. | Maziah M. | Abdullah M. P. | Abdullah M. Z.
Biochemical analyses were carried in an effort to assess the superior quality of rice seeds. Seeds of thirteen rice cultivars, IR64, MR84, MR151, MR159, MR167, analyzed for strach and protien content. In addition, ascorbic acid content and glutathione reductase (GR) activity were also determined for their antioxidative properties. The dry weight of seeds was recorded. The results obtained showed that seeds of cultivar RU 2242-1-1 contained the highest strach content (34.06 mg/g fr.wt)followed by MR209 (26.21 mg/g fr.wt) and IR 64 (24.07 mg/g). The seeds with low strach content are MR207 (18.11 mg, MR159 (18.48 mg/g) and Setanjung (18.73 mg/g). The level of strach present in the seeds may indicate the photosynthetic and strach synthesis efficiency of the cultivars. Seeds of MR221 exhibited the highest protien content (147.77 ug/g fr.wt) followed by MR84 (141.59 ug/g fr.wt) and MR159 (100.52 ug/g fr.wt) while seeds of cultivars MR207 26.63 ug/g fr.wt), MR167 (33.55 gu/g fr.wt) and Setanjung 37.39 (ug/g fr.wt) have low protien content. Ascorbic acid content, measured by the 2,6 dichlorophenol-indo-phenol (2,6-DCPIP) titration method, was observed to be highest in seeds of MR209 (1713 ug/g fr.wt), followed by IR64 (1395 ug/g fr.wt) and MR84 (1131 ug/g fr.wt), followed by IR64 (1395 ug/g fr.wt) and MR84 (1131 gu/g fr.wt). Seeds of RU 2242-1-1 showed the highest specific GR activity (20.68 nmol/min/mg protien), followed by MR219 (12.55 nmol/min/mg protien), Setanjung (11.71 nmol/min/mg protien), and IR64 (11.40 nmol/min/mg protien). The seeds dry weight was lowest in seeds of MR209 (3.00%), followed by MR151 (3.25%), RU2242-1-1 (3.50%) and Muda (3.50%). Low moisture content in seeds may reflect storage quality. From the biochemical parameters carried out, the cultivars that appear to have potential are MR209, RU 2242-1-1 and IR64. The seedlings produced from these seeds will be further evaluated to determine their quality and potential as superior seed stock.
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