Gene expression analysis of ATL31 in yellow corn in response to Philippine downy mildew infection
2019
Shaikh, M.L. | Lantican, D.V. | Pascual, C.B. | Zaporteza, M.M. | Manohar, A.N.C. | Pammit, F.K.L. | Garcia, M.O. | Gardoce, R.R. | Nuñez, J.P.P. | Aglibot, C.C. | Galvez, H.F.
Philippine downy mildew (PDM) caused by the obligate oomycete Peronosclerospora philippinensis (Weston) Shaw is one of the most destructive pathogens of corn in the Philippines. Through the years of using metalaxyl as seed treatment and foliar spray to mitigate the spread of the disease, metalaxyl-resistant downy mildew strains have evolved leaving host resistance as the most effective and efficient control measure. Previous fine-mapping effort has identified a major QTL region for corn PDM resistance and characterized to have high homology with corn ATL31 E3 ubiquitin ligase. To validate the role of ATL31 in the host resistance mechanism of corn to PDM, differential gene expression (DGE) analysis of ATL31 using qRT-PCR was performed between resistant and susceptible genotypes across two different time points (before and during PDM exposure). Selection of the resistant and susceptible genotypes for DGE was based on host resistance (HR) screening via spreader row technique. Result showed that the resistant genotype has a significant decline in the ATL31 gene expression as compared to that of the susceptible genotype, two weeks after PDM exposure. This is the first report of the rapid and transient expression of ATL31 and its possible role in basal immunity of yellow corn exhibiting resistance response to PDM, as previously observed in Arabidopsis. Such information on the ATL31 gene expression provides a glimpse on the complex host-pathogen interaction during PDM disease progression in corn. Thus, this study will provide a platform to design DNA marker tags for downstream marker-assisted plant breeding application towards development of outstanding and PDM-resistant corn varieties.
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