Rapid screening for climate-ready rice (Oryza sativa L.) and elimination of duplicates in the genebank
2019
Caguiat, X.G. | Duldulao, M.D. | Rañeses, M.A.M. | Ferrer, M.C.
Genebank serve as reservoir of traits that could be mined to produce rice varieties with tolerance and resistance to biotic and abiotic. There is a need for fast and reliable method to determine which among the vast germplasm kept in the Genebank could be used in breeding programs. Gene-specific SNP-marker based offers the possibility of rapid screening, diversity analysis, and possible elimination of duplicates in the Genebank. This study was conducted to: 1) screen two common rice germplasm in the Genebank having same names but with multiple collection, 2) determine if these markers could be used to eliminate duplicates and, 3) examine the level of diversity of entries with same accession name. Total of 123 'Palawan' and 71 'Kamuros' were used in the study. Results showed high levels of variation within and between the entries for 'Palawan' and 'Kamuros'. 'Palawan' entries have higher resistance to blast (49%) and more tolerant to submergence (1%). Overall diversity was slightly higher for 'Kamuros' (72%) than 'Palawan' (70%) however combined diversity at 67%. Some entries overlapped between the two accession, cluster analysis resulted to single entry to eight entries per subclustered in 'Kamuros' while 'Palawan' has as high as 18 entries per sub-cluster. The results could infer more duplicates in 'Palawan' than in 'Kamuros' and gene-specific markers could be used as rapid screening method for vast collection in the Genebank. Duplicate elimination could save more resources in germplasm management whereas molecular markers could fast-track the discovery of donor germplasm for developing climate-ready rice varieties. This study underscores the need for rapid screening of germplasm in the Genebank and elimination of their duplicates.
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