Optimization of an embryo rescue protocol in abaca (Musa textilis Nee)
2019
Vera, S.V.I. | Lalusin, A.G.
Abaca (Musa textilis Nee), a fiber crop endemic to the Philippines, has been in high demand by the global market for its tensile strength and quality. However, one of the challenges that breeding and production of abaca is facing is its low germination rate in soil. To address this, the use of in vitro techniques such as embryo rescue has gained the interest of many researchers. This study was conducted to optimize an embryo rescue protocol for two abaca hybrid genotypes, BC2-2 and BC2-7. Developmental parameters which include percent germination, days to germination, number of leaves per explant, incidence of multiple shoot formation and plant height for each hybrid were observed. Two hundred and forty (240) embryos of each genotype were inoculated on media with four varying kinetin concentration. The control treatment consists of the basal medium with no kinetin supplementation, while treatments 1, 2 and 3 were augmented with 0.1 mg L-1, 0.3 mg L-1 and 0.5 mg L-1 kinetin, respectively. For BC2-2, all parameters except for the days to germination, were found to be best in treatment 3. For BC2-2, it was concluded that the performance of embryos improved as kinetin concentration is increased while for BC2-7, only an optimum level of kinetin that is 0.1 mg L-1 worked best.
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