Not all sequences found in the internet are based on correctly identified specimens: examples from data analyses for stick insects (Phasmatodea) and other examples
2018
Abenis, K.O. | Lit, I.L., Jr | Doo-Sang Park | Eusebio, O.L.
The advancement in technologies, especially tools for molecular techniques, continually evolves, thus reducing costs for basic analysis like DNA barcoding. This taxonomic method that uses a short genetic marker in an organisms' DNA is a widely used tool for identifying organisms by many researchers at present. This method has confirmed morphological phylogenies of many groups, and even resolved species-complex problems. A few cases in the Philippines were even published as new species based solely on molecular data. However, the research on stick insects (Phasmatodea) shows that despite having stick insect sequences deposited in databases like GenBank and BOLD Systems, matches to query cover may hit to a hundred percent but identifies our specimen to a different genus. For instance, Pharnacia ponderosa Stal (Phasmatidae: Clitumaninae) is identified as Neohirasea japonica (Haan) (Lonchodidae: Necrosiinae) in GenBank but barcodes for P. ponderosa are likewise available in the gene banks. Researchers' case for the wasp (Hymenoptera, Ammophila coronate A. Costa also had 100% query cover to family Tarsonemidae (Acari) and Oenochroma vinaria Guenee (Lepidoptera).
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