Identification of allexivirus and potyvirus from the different garlic varieties of the three major grousing regions in the Philippines using RT-PCR [reverse transcription polymerase chain reaction]
2019
Plaza, R.U. | Espino, M.R.M. | Espino, R.R.C.
Garlic (Allium sativum) is an economically important crop in the Philippines. Region 1 [Ilocos Region], Region 2 [Cagayan Valley] and Region 4-B [Mindoro, Marinduque, Romblon, and Palawan] are the three major growing regions crop in the country. All varieties of garlic had been found to be infected by different viruses. This had resulted in small bulb formation resulting to low yield. In this study, eight garlic varieties from the different farm areas of the 3 regions were assessed for the presence of Allexvirus and Potyvirus specifically Onion Yellowing Dwarf Virus (OYDV) using gene specific primer from the coat protein and untranslated region of viral RNA through transcription polymerase chain reaction or RT-PCR. The product for allexi and OYDV was observed at approximately 200 bp and 318 bp, respectively confirming the positive isolation of the viral RNA. In addition, incident rate of virus infection of allexivirus and OYDV varies from the different farm areas on each region. Garlic varieties form Batanes and Occidental Mindoro showed the highest incident rate on allexivirus infection, while varieties from Pangasinan and Occidental Mindoro were the highest for OYDV. Further collection are being undertaken in other garlic growing areas to determine the presence and extent of infection of these viruses. This will provide useful information as to areas where sourcing of planting materials of garlic can be obtained for future planting.
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