Gene-specific marker characterization of Philippine traditional rice varieties
2019
Alvarino, J.B. | Mananghaya, T. | Noines, J.
Molecular characterization is a significant tool in mining of valuable traits in rice germplasm gene pool. Molecular markers are associated with specific genes and functions. These markers act as 'signposts' with those genes that gives a visual direction to crop improvement program. Also, it creates molecular profiles of the germplasm, and identify potential novel and economically important traits. Gene-specific trait molecular characterization through microsatellites has been routinely done in PhilRice [Philippine Rice Institute]. Isolated genomic DNA of 1,400 Traditional Rice Varieties (TRVs) accessions from PhilRice-Gene bank was characterized using functional markers on the following: xa5 marker for bacterial leaf blight; RM6152 and RM6403 markers for detection of Rice Tungro Virus resistance gene, ART5 and SC3 markers for submergence; RM511 for drought tolerance, RM8094 and RM10793 for saline resistance and; BADH2 and Bradbury multiplex markers for fragrance gene on aromatic trait. Most of the gene specific trait markers are detected and analyzed based on presence and absence of those genes. We identified rice varieties from the currently available 5,000 accessions in Genebank which poses a multiple genes or associated to above traits.
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