Marker assisted introgression of provitamin A trait into popular rice varieties
2019
Amparado, A.F. | Samia, M.Q. | Fabro, A.M. | Delos Reyes, K.A.B. | Inabangan, M.E.S. | Dimaculangan, C.A. | Punzalan, J.D. | Morales, G.M. | Estonilo, R.C. | Micosa, F.R. | Bautista, A.M. | Boncodin, R.M. | Reinke, R. | Swamy, B.P.M.
Hidden hunger in the form of vitamin-A deficiency (VAD) is prevalent in developing countries worldwide. Around 2.1 million children are suffering from this deficiency in the Philippines. The development of golden rice is seen as a possible intervention in mitigating this problem. Introgression of the golden rice gene (GR2E) into Philippine rice varieties would ensure its successful adoption and consumption by the target population in the Philippines. With the use of market-assisted backcrossing, introgression of GR2E into PSBRc82 was carried out at IRRI-HQ. A series of back crossing of GR2E trait resulted in a successful introgression of GR2E gene into PSBRc82 that generated advanced progenies with carotenoid content ranging from 5.0-7.0ppm and phenotypic, yield and yield component traits comparable with the recipient parent PSBRc82. Five promising lines were identified for further evaluation in multi-environment trials in the Philippines. Using the same method, additional popular Philippine rice varieties namely PSBRc18, NSICRc222, NSICRc238, NSICRc302 and NSICRc356 were successfully converted into GR2E golden rice. Each of these materials is in advanced stages of breeding and ready for field testing.
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