Determination for usability of grapevine bleeding water as in vitro nutrient media

In this study, it was tried to determine whether utilized for nutrient media of grapevine bleeding water which has a very rich content in plant tissue culture studies by comparative with 5 commercial nutrient media. Bleeding water was collected from "Karaerik (Cimin)" and "Erciş" grape cultivars at the pruning period. Essential nutrient media were added as indicated amounts per liter and the pH was adjusted to 5.7 in all media. 30 g B5 was added into MS and SH medium with some bleeding water. Similarly, 20 g sucrose was also added into WPM medium with some bleeding water. However, WH medium was composed only some bleeding water without sucrose. After addition of agar (0.8%), all media were sterilized in the autoclave at 121 ˚C for 20 minutes. Pieces of leaf palm, petiole and nodes which were obtained by shooting of cuttings taken from both cultivar at the pruning period were used as explants. Each explant was planted in petri dishes as 10 pieces in per dish. Cultures were incubated with 8/16 h photoperiod at 2000 lux light and 25 °C for four weeks. The explant rates to form adventitious shoots and callus as well as callus quality (type I and type II) (%), and different callus degrees were examined at the end of the four weeks. Routine analysis such as EC, pH, TA%, and TSS% as well as spectrophotometric assays: total phenolic and total antioxidant were executed. In addition, minerals were also analyzed by ICP-OES device. Cultured explants showed different growth level in the different media. As conclusion, it can be stated that bleeding water in grapevine can be utilized directly as tissue culture media. In addition, it is likely that bleeding water can be also used as source in tissue culture study like coconut milk which is unidentified as chemically.