High-Throughput Conformation-Sensitive Gel Electrophoresis for Discovery of SNPs
2001
Y.F. Leung | P.O-S. Tam | W.C. Tong | L. Baum | K.W. Choy | D.S.C Lam | C.P. Pang
High-throughput screening for single nucleotide polymorphisms (SNPs) or mutations can be achieved by inexpensive technologies. We modified the original protocols of conformation-sensitive gel electrophoresis (CSGE) to increase throughput several fold to 1.3 samples/min, which is about five times faster than denaturing high-performance liquid chromatography (DHPLC). The modifications include decreasing the gel thickness, increasing the number of lanes to 96, and increasing the number of samples per lane to seven. This high-throughput CSGE method is fast, robust, and as simple as the original protocols. Together with a two-stage strategy for screening homozygotes and the replacement of ethidium bromide with SYBR® Gold DNA dye staining, this protocol is a reliable and cost-effective alternative for laboratories that require high-throughput screening.
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