Molecular characterization and VP1 sequence analysis of Foot and Mouth disease new virus strains isolated during 2015-2017 in Beni-Suef, Egypt
2018
Amany T. El-Gendy | Abdel-Hamid Bazid | Hanafy M. Madbouly | S.M.Tamam
Outbreaks of Foot and Mouth Disease (FMD) cause severe economic loss tothe livestock industries in terms of loss of meat, milk production and the highmortality rateespecially in calves. This study was intended for detection,isolation and molecular characterization of FMDV circulated strain amongdifferent regions in Beni-Suef governorate, Egypt during 2015 and 2017. Onehundred field samples were collected from clinically diseased cattle andbuffaloes including vesicular fluid and sloughed epithelial membrane.Molecular detection and differentiation of FMDV serotypes by RT-PCRshowed that (71%) were positive for serotype O indicating its predominance inBeni-Suef, Egypt.Also (18%) were positive for serotype SAT2 and (7%) wereun-typed serotype being only positive for universal primer. Twenty sampleswere isolated on BHK-21 cells clone 13. Three samples showed thecharacteristic CPE of FMDV after blind passage 4 times. Sequences of VP1coding-region of the three isolated FMD virus showed that the three isolatedviruses were serotype O. The phylogenetic analysis of the isolated virusesreveled that they were closely related to type O of those reported in Ismailia,Behira, Giza and Cairo during 2016 with identity ranged from 97.7% - 99.8%.The most relevant outboard isolate was SUD/8/2008 with 93% identity afterBlast homology search. However, the phylogenetic tree analysis revealed thatthe three FMDV type O isolates FMDV/serotype O/Beni-Suef/2017 differspartially from all other Egyptian serotypes of 2016. In conclusion, serotype Owas the most prevalent FMDV serotype in Beni-Suef, Egypt.
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