Detection and Identification of Helicobacter pullorum in Poultry Species in Upper Egypt
2014
Ahmed K. Hassan | Mostafa A. Shahata | Elrefaie M. Refaie | Ragab S. Ibrahim
This work aimed to detect, identify and study the epidemiology of Helicobacter pullorum in avian species in Upper Egypt. A total of 1800 samples, including cloacal swabs, cecal swabs and livers were collected from chickens, turkeys and ducks in five different governorates in Upper Egypt. Using conventional phenotypic methods for isolation and identification, Helicobacter pullorum could be identified only from chickens with percentage 39.33% and no presence of the organism in turkeys and ducks. Sixteen randomly selected phenotypically identified Helicobacter pullorum isolates were confirmed using PCR assay based on 16S rRNA gene. In conclusion, conventional phenotypic methods for detection and differentiation of Helicobacter pullorum are often hampered by many limitations, while molecular methods, and PCR, in particular, have marked an important step forward in bacterial diagnostics and can provide a sensitive and rapid alternative method for detection and identification and highlights the potential of PCR technology in routine detection and identification of pathogens.
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