Effect of the Enzymatic Fungal Extract of Pochonia chlamydosporia on the Viability of Fasciola hepatica eggs

Lorena Souza Castro; Isabella Vilhena Freire Martins; Victor Menezes-Tunholi Alves; Fernanda de Paula Roldi Vieira; Gabriella Peterlini Tavares; Jackson Victor Araújo

Effect of the Enzymatic Fungal Extract of Pochonia chlamydosporia on the Viability of Fasciola hepatica eggs

2020

Hepatic fasciolosis has been implicated as one of the most important parasitic diseases affecting the sanity of cattle and sheep, reflecting in significant economic losses besides being an important anthropozoonotic disease. The eggs of Fasciola hepatica are eliminated with the feces of these hosts, and under the influence of extrinsic and intrinsic factors may remain in the environment for months until they find favorable conditions for embryogenesis. The objective of the present study was to evaluate the viability of Fasciola hepatica eggs exposed for 60 minutes at different concentrations of the enzymatic extract of Pochonia chlamydosporia (Pc-10). Subsequently, they were sedimented and placed on 24-well plates containing the extract of P. chlamydosporia (Pc-10) at concentrations of 500 μL (10%), 400 μL (8%), 300 μL (5%), 200 μL (2%), 100 μL (1%) and distilled water (control group). The experiment was carried out in triplicate, using a total of 900 eggs. After the experimental exposure to the enzyme extract of the fungus (Pc-10), 98% of the eggs exposed to the enzymatic solution demonstrated significant ultrastructural alterations in their respective teguments, when observed in scanning electron microscopy and transmission. The ultrastructure showed a collapse of the internal walls of the egg, interfering in the opening of the operculum. The use of the enzymatic extract of P. chlamydosporia (Pc-10) compromised both the external tegument, breaking it and pasting it, as well as vital structures of the embryonic activity of the F. hepatica eggs.

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