STUDIES ON FISH liPIDS II. FATTY ACID COMPOSITION QF LIPIDS OF Oil SARDINE (SARDINELLA LONGICEPS)
2016
GOPAKUMAR, K. | RAJENDRANATHAN NAIR, M.
Gas-liquid chromatography has been employed for the qualitativeand quantitative analysis of the component fatty acids in lipidsof oil sardine (Sardinella longiceps). Phospholipids and triglyceridesof the lipids were previously separated by column chroma·tography before they were converted into the methyl esters ofthe fatty acids. The predominant acids present in the depot fat ofthefishhavebeenfoundtobeC 14:o = 8.13%, C16:o = 27.9%,C18:o = 3.8%,C18:1 = 15.4%. C20 :5 = 10.6% andC22 :6 =8.8%.Apart from the above acids the .distribution of minor acidsbelonging to cl81 c2 0 and c2 2 groups have also been workedout. The separated phospholipid fraction contained more than 70%polyunsaturated acids of which the important constituents weredocosahexaenoic (C2 2 :6 =28%) and eicosapentaenoic (C2 o:s· =10.6%). A marked reduction was found in the amounts ofpolyunsaturated acids in triglycerides, their total amount registeringabout 20 percent. This fraction recorded about 48 per cent of C16acids of which palmitic and palmitoleic acids amounted to 25.8per cent and 19.1 per cent respectively. Occurrence of oddnumbered fatty acids c15 and cl 7 has also been noted in thephospholipid and. composite samples of the fish.
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