Genetic diversity of Colletotricum truncatum infecting urdbean (Vigna mungo) in Himachal Pradesh
2016
ABHISHEK KATOCH, DAISY BASANDRAI | PREM NATH SHARMA, ASHWANI KUMAR BASANDRAI*, VARUN SHARMA,
Urdbean is an important pulse crop of India and an assured kharif pulse crop of Himachal Pradesh. The crop is vulnerable to many diseases, out of which anthracnose caused by Colletotrichum truncatum takes a heavy toll of the crop. The disease may be effectively managed by the cultivation of resistant varieties and development of such varieties depends on availability of effective resistant sources and variability aspect of the pathogen. Hence, in the present studies 31 isolates of C. truncatum were characterized into six pathogenic races based on reaction on indigenous differential set. Pathogenic race Ct I accommodated 11 isolates and was virulent on four differential genotypes whereas, race Ct III was least virulent. Genotyping of the different isolates using inter simple sequence repeat marker (ISSR) grouped 31 isolates into six major clusters at 70 per cent similarity coefficient using NTSYS software/version 2.0. Though there was sampling biasness with more isolate representation from major urdbean cultivating area Sirmour, but four isolates (Ct 27, Ct 28, Ct 30 and Ct 31) were found early divergent in genetic analysis. Thus, indicting restricted gene flow in these geographic pockets. Moreover, prevalence of all major pathogenic forms of C. truncatum except race Ct III from Sirmour district suggests that this area could be harnessed as hotspot for screening resistant genotypes in vivo.
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