Impact of priming and storage containers on enzyme activities of naturally aged seeds of Barley (Hordeum vulgare L.)
2022
Siddu Appasaheb Kurubar | Bhuker, Axay | Sukham Madaan
The study was conducted on six barley varieties viz. BH 885, BH 946, BH 393, BH 902, DWRB 92 and DWRB 101 at CCS HAU, Hisar during 2020-21. The results revealed that maximum catalase activity (243.168 and 157.981umoles/g FW), peroxidase activity (22.33 and 35.03 units/g FW), superoxide activity (22.03 and 35.03 nmoles/g FW) and dehydrogenase activity (0.213 and 0.245 OD/g/ml) were estimated in the seeds primed with ZnO NPs @ 100ppm in cloth and polythene bag, respectively. Among the varieties, DWRB 92 recorded highest catalase activity (217.168 umoles/g FW) in cloth bag while BH 393 recorded maximum catalase activity (232.961 umoles/g FW) in polythene bag. Minimum catalase activity was observed in DWRB 101(60.351 umoles/g FW) in cloth and BH 885 recorded least (121.418 umoles/g FW) in polythene bags. Maximum peroxidase activity (21.22 and 31.56 units/g FW) was estimated in BH 885 while minimum (12.71 and 22.54 units/g FW) in BH 946 in cloth and polythene bag, respectively. Maximum SOD activity (18.033 and 25.033 nmoles/g FW) was measured in DWRB 92 and minimum (12.712 and 22.537 nmoles/g FW) in BH 946 in cloth and polythene bag, respectively. Maximum DHA activity (0.170 and 0.206 OD/g/ml) was recorded in DWRB 101 and least in BH 946 (0.234 and 0.281 OD/g/ml) in cloth and polythene bag. It is concluded that seed quality can be maintained through priming with ZnO NPs@100ppm at 25°C for 24 hours. Barley seed quality can be maintained by storing the seeds in polythene bags (>700gauge) with optimum moisture content (<8%).
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