Copper tolerant bacteria and fungi isolated from copper contaminated agricultural soil
2011
Che Fauziah I. | Siti Nazurah Mohd Kamel | Radziah Othman
This study aimed to isolate and identify potential copper-tolerant fungi and bacteria from copper contaminated vegetable sites. Soils samples were analysed for Cu content using aqua regia digestion, together with corresponding plants samples using nitric acid digestion. Fungal and bacterial strains were isolated from soil and surfacesterilised plant roots. Copper tolerance was measured by in vivo assay using dilution technique and isolates were identified based on 16S rDNA gene sequence analysis. The Minimum Inhibitory Concentrations (MICs) ranged from 50 to 300 mg Lˉ¹ depending on the isolates which belonged to the genera Trichoderma (UPMF2), Penicillium (UPMF3), Fusarium (UPMF4), Aspergillus (UPMF5), T.viride (UPMF6), Bacillus spp. (UPMB2) (UPMB7), Bacillus cereus (UPMB4), Burkhoderia sp. (UPMB5), Seratia marcesens (UPMB6) and Bacillus mojavensis (UPMB8). Bacillus sp. (UPMB7) and T. viride (UPMF6) isolates were the most Cu tolerant and exhibited strong growth. The merits of these strains as potential candidates for bioremediation require further investigations.
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