Isolation of ligninase producing Basidiomycetes from local terresterial
2011
Tay, Amelia ChuFer | Yong, Suh Kuan | Tee, Jiun Tsong | Ong, Lisa Gaik Ai | Chan, Mun Yee | Hii, Siew Ling
Synthetic dyes have replaced the use of natural dye in the textile industry. Generally, the synthesized dyes are more colourful, easy to manufacture, resistant to fading and have a diverse range of colour. Despite all these mentioned advantages, this group of dye has caused water pollution in Malaysia, especially in the state of Terengganu and Kelantan. They have caused aesthetic pollution of waterways, due to the presence of dyes leached from textile factories since they are visible even in trace amounts. Ligninase produced by basidiomycetes is able to degrade azo, heterocyclic, reactive and polymeric dyes which have been found abundant in the wastewater. This enzyme is further subdivided into laccase, lignin peroxidase and manganese peroxidase. Thus, fungal isolated from Perak and Selangor were tested for ligninolytic enzyme production on plates containing 3 commercial dyes. Of the tested isolates, a total of 8, 13 and 11 isolates showed positive for 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), Remazol Brilliant Blue R (RBBR) and guaiacol, respectively. Isolates positive for all three dyes were subjected to morphological identification by using lactophenol staining.
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