Comparison of sample preparation methods and chemical assays for determination of titanium, phosphorus and calcium in broiler diets and digesta
2020
Samat N. | Miller, H.
In understanding the effectiveness of phytase in improving nutrient digestibility, digesta samples from each segment of the gastrointestinal tract (GIT) need to be analysed for several different nutrients which require a large amount of dry matter. This study aimed to develop a typical sample preparation that could minimize the amount of digesta samples required and enable sequential chemical analyses. The specific objectives were to compare the content of titanium (Ti), phosphorus (P) and calcium (Ca) in diet and digesta samples after being separately prepared using different acid digestion and analysed by different chemical methods. In this study, gizzard and terminal ileal digesta were collected from 20 d old Ross 308 male broilers fed with wheat/corn diets either with or without supplemental phytase at 1500 FTU/kg. Diets and digesta samples were digested in hydrochloric acid (HCl), and sulphuric acid (H2SO4) and the contents of Ti, P and Ca were determined by colourimetry and ICP-OES methods. The present study showed that H2SO4 could be used to replace HCl in sample digestion before mineral analysis. Furthermore, digestion of diet and digesta using H2SO4 enabled sequential analysis of Ti, P and other minerals.
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