Anti-human Papillomavirus (HPV) type 16 E6 activities in Ganoderma lucidum
2010
Lai, L. K. | Nurhayati Z. A. | Noorlidah A.
The development of cervical cancer is reported to be associated with human papillomavirus type 16 (HPV 16). The E6 region of HPV 16 encodes for the E6 oncoprotein which is involved in cellular transformation of the cervical epithelia leading to neoplasm. The E6 oncoprotein is abundant in cervical cancer and cervical cancer-derived cell lines. Realising the importance of the E6 oncoprotein in the development of cervical cancer, the present study was undertaken to screen for Ganoderma lucidum extracts for the suppression of the E6 oncoprotein. In this study, dichloromethane, ethanol, water and polysaccharide extracts of G. lucidum were analysed for anti-HPV 16 E6 activity in the HPV 16-containing cervical cancer-derived cell line, CaSki. The extracts were first incubated with the CaSki cells for 72 hours. A polymer-based immunocytochemistry technique was then performed on the cells to analyse the expression of the E6 oncoprotein. Qualitative observation showed that expression of E6 oncoproteins reduced in CaSki cells incubated with the extracts of G. lucidum indicating anti-HPV 16 E6 activities in these extracts. When compared, the dichloromethane extract demonstrated the strongest anti-HPV 16 E6 activity. The dichloromethane extract was subsequently subjected to thin layer chromatography (TLC) to evaluate the profile of secondary metabolites present in the extract. When evaluated, the profiling showed the presence of terpenoids and alkaloids. This study indicated that G. lucidum is a potential treatment for cervical cancer.
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