Efficacy of silvernano bacterial cellulose insole on Micrococcus sedentarius growth
2019
Sitlaothaworn, Kanchana | Dechkla, Manussawee
Silver nanoparticles (Agº) impregnated into bacterial cellulose (BC) by immersion in an antimicrobial concentration of 1,700 µg/ml (10 mM) AgNº3 solution were used to synthesize silver nanoparticle-impregnated bacterial cellulose (Ag0 BC) composites. Agº BC incorporated into insertable footpad was developed for reduction of foot odor associated with pitted keratolysis, primarily caused by Micrococcus sedentarius. A scanning electron microscope (SEM) revealed the porous structure of AgºBC fibers impregnated with Agº particles. The antibacterial activity of AgºBC for Micrococcus sedentarius revealed a growth inhibition zone of 30 mm in diameter by disc diffusion method. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of AgNº3 solution against Micrococcus sedentarius were 0.42 µg/ml and 0.83 µg/ml, respectively. The effficiency of an AgºBC footpad applied to shoe samples was measured via total bacterial counts over a time period of 14 days. As expected, the percentage of surviving bacteria in three different types of shoe samples was gradually decreased in the period beginning on the 2nd day. Moreover, no bacterial growth was found in any shoe sample within 8–14 days. However, the cytotoxicity of 5.31–1,700 µg/ml AgNOз concentrations revealed 50% growth inhibition (IC₅₀) on human dermal fibroblast cells by resazurin microplate assay (REMA). Thus, it can be concluded that Ag0 BC, synthesized with lower doses of AgNO3 concentration (< 1,700 µg/ml), may be able to apply in Agº BC footpad production for inhibition of Micrococcus sedentarius growth and prevention of foot odour in different types of footwears.
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