Assessment of the microbial diversity in soils using conventional and molecular technologies
2020
Ocampo, E.T.M. | de los Reyes, A.M.M. | Fulleros, M.C.A.
Soil microorganisms are highly diverse and perform multiple and beneficial roles in soil ecosystems. However, the study of microbial communities is inherently difficult because only one percent of microorganisms grow in culture. Hence, there is need to explore techniques that result in identifying microbial strains and, consequently, the diversity of microorganisms in a certain community. This chapter presents various techniques ranging from the conventional, culture-dependent methods that are molecular or genetic-based. Included also in the discussion are easy-to-follow and highly specific methodologies from soil collection, microbial isolation, DNA extraction, molecular and PCR analysis, and statistical procedures. Microbial DNA extraction for upland and lowland paddy are presented. The study of soil metagenomes using molecular markers and metagenomics is also discussed.
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