Polymorphism of prolactin (PRL) and beta-lactoglobulin (Β-LG) genes in the Lacaune sheep and their relation-ship with milk productivity
2021
Evlagina, Daria D.
Introduction. Dairy sheep breeding in Russia is a new and understudied area. Prolactin and beta-lactoglobulin genes are known to be the genes controlling milk production in many mammalian species, including sheep. The aim of the study was to examine the effect of DNA marker genotypes of prolactin and beta-lactoglobulin genes on the sheep milk productivity. Materials and methods. The study focused on the formed groups of the Lacaune sheep (n=248) bred at Nikolaev farm enterprise, Krymsky district, Krasnodar Krai. The gene polymorphism was determined through PCR-RFLP analysis. The amount of milk for 180 days of lactation was measured individu-ally by carrying out control milking every 14 days. Qualitative parameters of milk – the content of fat and pro-tein – were determined using Laktoscan M analyzer according to the study protocol. Results, discussion. This study reveals that the polymorphism of the PRL and β-LG genes is represented by the following alleles: PRLА, PRLB; β-LGА, β-LGВ and PRLAA, PRLAB, PRLBB, β-LGAA, β-LGAB, β-LGBB genotypes, respec-tively. The PRLA allele was found to have a high frequency of 0.81 and the PRLB allele has a low frequency of 0.19. The β-LGB allele frequency amounted to 0.66, while the β-LGA frequency was 0.34. The animals with ho-mozygous genotypes PRLАА and β-LGАА had better milk yield per lactation. The ewes with homozygous geno-types PRLВВ and β-LGВB had more fat and protein in their milk than their counterparts. Conclusion. The results obtained indicate the expediency of using as an additional criterion the selection of Lacaune sheep with the PRLBB, β-LGBB genotype for milk production with the aim of its further processing into cheeses.
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