Supercritical CO2 Extraction of Phytocompounds from Olive Pomace Subjected to Different Drying Methods
2021
Graziana Difonzo | Antonella Aresta | Pietro Cotugno | Roberta Ragni | Giacomo Squeo | Carmine Summo | Federica Massari | Antonella Pasqualone | Michele Faccia | Carlo Zambonin | Francesco Caponio
Olive pomace is a semisolid by-product of olive oil production and represents a valuable source of functional phytocompounds. The valorization of agro-food chain by-products represents a key factor in reducing production costs, providing benefits related to their reuse. On this ground, we herein investigate extraction methods with supercritical carbon dioxide (SC-CO2) of functional phytocompounds from olive pomace samples subjected to two different drying methods, i.e., freeze drying and hot-air drying. Olive pomace was produced using the two most common industrial olive oil production processes, one based on the two-phase (2P) decanter and one based on the three-phase (3P) decanter. Our results show that freeze drying more efficiently preserves phytocompounds such as &alpha:-tocopherol, carotenoids, chlorophylls, and polyphenols, whereas hot-air drying does not compromise the &beta:-sitosterol content and the extraction of squalene is not dependent on the drying method used. Moreover, higher amounts of &alpha:-tocopherol and polyphenols were extracted from 2P olive pomace, while &beta:-sitosterol, chlorophylls, and carotenoids were more concentrated in 3P olive pomace. Finally, tocopherol and pigment/polyphenol fractions exerted antioxidant activity in vitro and in accelerated oxidative conditions. These results highlight the potential of olive pomace to be upcycled by extracting from it, with green methods, functional phytocompounds for reuse in food and pharmaceutical industries.
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