Isolation of ripening-related genes of Capsicum annuum via a differential display technique
2006
Roslinda S. | Zamri Z. | Ismanizan I.
A mRNA differential display was established to allow the isolation and identification of genes induced during chili fruit ripening grow under normal conditioned. Twenty eight PCR-fragments from the differential display were identified using twenty four primer combinations. Only four cDNA fragments were successfully cloned and sequenced Database searchesrevealed that CUKMD1 encodes -carotene desaturase and CUKMD2 was identified as encoding O-methyltransferases. The fragment CUKMD3 shares 100% homology at amino acid level to catalase 1solated from (Capsicum annuum. While CUKMD4 showed considerable homology with an amino acid transporter. These studies showed that mRNA differential display is a powerful tool to facilitate the isolation and identification of genes induced during chili fruit ripening.
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