In vitro direct and indirect regeneration of plants from nodal and petiole explants in Pelargonium odoratissimum (L.) Herit.
2022
Asghar EBRAHIMZADEH | Maliheh FATHOLLAHZADEH | Mohammad Ali AAZAMI | Mohammad Bagher HASSANPOURAGHDAM
<p class="042abstractstekst"><span lang="EN-US">Nodal and petiole explants were employed to study the direct and indirect regeneration from <em>Pelargonium odoratissimum</em> <em>in vitro</em>. Direct shoots regeneration of nodal segments was tried in MS medium containing 1 and 2 mg l<sup>-1</sup> BAP and 0.5 mg l<sup>-1</sup> IBA. The highest mean shoots number and the greatest shoots per explant number were obtained in the medium containing 2 mg l<sup>-1</sup> BAP. Nodal segments were the source of petiole explants and the resulting petioles were cultured in ½ MS medium supplemented by 1, 1.5, 2 and 4.5 mg l<sup>-1</sup> BAP enriched with 0.1, 1 and 1.5 mg l<sup>-1</sup> NAA. With the petiole explants, the lowest browning percentage, the highest callus induction and also, the top number of shoots per explant were recorded in 2 mg l<sup>-1</sup> BAP + 0.1 mg l<sup>-1</sup> NAA medium. The medium supplemented with 0.2 mg l<sup>-1</sup> NAA exhibited the desired effect on rooting percentage and mean root number and length. The rooted young plants were transferred to the pots containing peat-moss and perlite (1:1) and the acclimatization was successful since, more than 90 % of plants adapted-well in the greenhouse conditions. This <em>in-vitro</em> propagation methodology would be advisable to the plant production systems and to whom wish to produce the clonal homogenous plants for the commercial ideas and for the detailed molecular studies.</span>
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