Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of Fridericia chica Leaves on Human Cancer Cell Lines Exposed to α- and β-Zearalenol

Fridericia chica (Bignoniaceae) is a Colombian Caribbean plant with numerous health benefits, including properties such as wound healing, immune system stimulation, and antioxidant capacity, among others. Mycotoxins alpha-zearalenol (&alpha:-ZEL) and beta-zearalenol (&beta:-ZEL) are phase I metabolites of zearalenone, a natural product involved in endocrine disruption and cell proliferation processes. This study aimed to investigate the cytotoxic potential of the hydroethanolic extract of F. chica leaves (HEFc) and determine their protective effects against proliferation induced by &alpha:-ZEL and &beta:-ZEL on human hepatoma HepG2, lung cancer Calu-1, and primary normal human epidermal keratinocytes, neonatal (HEKn). The cytotoxicity of HEFc was measured in a range from 4 to 1000 &micro:g/mL and from 0.4 to 100 &mu:M for both &alpha:-ZEL and &beta:-ZEL. Cell production of intracellular ROS was monitored using the H2-DCFDA probe. The cells exposed to HEFc presented IC50 of 128, 249, and 602 &micro:g/mL for the HepG2, Calu-1, and HEKn cells, respectively. A greater selectivity was seen in HepG2 cells [selectivity index (SI) = 3.5] than in Calu-1 cells (SI = 2.4). Cells treated with mycotoxins remained viable during the first day, and cell proliferation increased at low tested concentrations (0.4-6.3 &micro:M) in all three cell lines. However, after 48 h treatment, cells exposed to 50 and 100 &micro:M of &alpha:-ZEL and &beta:-ZEL displayed decreased viability. HEFc at 16 &micro:g/mL was able to give some protection against cytotoxicity induced by high concentrations of &beta:-ZEL in HepG2, reducing also cell proliferation elicited at low levels of &alpha:-ZEL and &beta:-ZEL. ROS production was not observed in cells treated with this HEFc concentration: however, it prevented ROS formation induced by treatment with 50 &micro:M &alpha:-ZEL or &beta:-ZEL. In summary, HEFc isolated from plants grown in northern Colombia displayed promising results against cell proliferation and oxidative stress caused by mycotoxins.