Effects of levamisole dilutions on HA properties of newcastle disease virus vaccine
1981
Prachak Poomvises | Achara Tawatsin
Effects of levamisole dilutions on HA of Newcastle Disease virus were studied in vitro and the findings are: 1. Treatment period 4 hours (at room temperature) - 1.1 When levamisole were mixed up to make final dilutions of 0.02 and 0.05% with NDV, no effect on HA was apparent; 1.2 When the final dilutions were 0.1, 0.25, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0%, the HA dropped significantly; 1.3 When the final dilution were 4.5 and 5.0% all HA activities of the NDV were destroyed. 2. At final dilution 0.02% levamisole did not affect the HA when the NDV were treated for 24 hours at room temperature. 3. HA activities of NDV was usually affected when NDV were treated longer than 4 hours when the final dilutions of levamisole were higher than 2.0%. Levamisole dilution should not exceed 0.1% when it is used as ND Vaccine diluent.
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