Induction and characterization of pKM101 mutants in Salmonella typhimurium
1982
Baik, H.S. | Kang, S.H. | Lee, S.Y. (Korea Univ., Seoul (Korea R.). Coll. of Agriculture, Dept. of Agricultural Chemistry)
Mutants of plasmid pKM101 modified to enhance mutagenesis were induced and characterized in Salmonella typhimurium. The pKM101 mutant plasmid were transfered normally and maintained in cells. They had modified in their ability to enhance the reversion of both point and frameshift mutations, to protect the cell against UV-irradiation and chemical mutagen treatment, of ampicillin resistance. A similar modification in enhancement of reversion was also observed in a urvB-strains. These results indicated that mutator effect of pKM101 was coded by one plasmid gene
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