A green house technique for screening faba beans (Vicia Faba L.) for resistance to Orobanche spp.
1985
Kukula, S.T. | Masri, H. (ICARDA, Aleppo (Syria))
Field screening of faba bean genotypes for resistance to Orobanche is often unreliable because of uneven distribution of Orobanche seeds in the soil and the interference of environmental factores in the development of the parasite. For a rapid screening of large number of faba bean genotypes, a greenhouse technique was developed. The technique consists of growing test genotypes in a greenhouse (20-21 deg. C), in polyethylene bags, using attapulgus clay as the growth medium, artificially infested with Orobanche seeds. The root system of the plants is examined at flowering (about 65 days after planting) for haustoria attachment after removing the clay. The number of germinated seeds of Orobanche is also counted to get a picture of host genotypes difference. Using this technique, differences in resistance of 15 genotypes of faba bean to Orobanche have been described
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