Studies on plant regeneration from mesophyll protoplasts of Solanum tuberosum L.
1988
Li Gengguang | Zhang Lanying (Academia Sinica, Guangzhou (China). South China Inst. of Botany)
Protoplasts from potato mesophyll of two strains were induced to callus in culture medium of protoplasts. The callus derived from mesophyll protoplasts were transferred to MS medium with 2 mg/l ZT+0.1mg/l IAA. Shoots regenerated from the callus were detected after 70 days of culture. The shoots which had grown to a height of 2-3cm were transferred to MS medium with 0.05 mg/l NAA. Roots were coming out in a few days. Complete plantlets were achieved. Stem segments with 1-2 leaves were then transferred to a mixture of sterilized soil and grown, and produced tuber.
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