Diagnostics of ovine sarcocystosis by IFAT (indirect fluorescent antibody test) and ELISA
1992
Svobodova, V. | Nevole, M. (Vysoka Skola Veterinarni, Brno (CSFR))
The indirect fluorescent antibody test (IFAT) was compared with the enzyme-linked immunosorbent assay (ELISA) for the detection of specific antibodies to Sarcocystis sp. A set of 275 ovine blood samples was examined by both reactions. Cystozoites of Sarcocystis gigantea were used as the corpuscular antigen for the IFAT. For the diagnostics of sarcocystosis by the ELISA technique the sandwich test of the antibody titration with a soluble antigen which was also prepared from S. gigantea macrocysts was used. Our studies confirmed that this antigen did not cross-react with Toxoplasma gondii. Titre 40 was determined as the limit one for the IFAT and titre 80 for the ELISA which was confirmed by the direct detection of cysts in the muscles. 76.7 % of the blood samples reacted positively in the IFAT and 83.6 % in the ELISA. These methods were found to be suitable and can be utilized for the intravital routine diagnostics.
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