Chitosan production from carapace and shell of black tiger shrimp (Panaeus monodon)
1993
Soottawat Benjakul | Pairat Sophandora (Prince of Songkla Univ., Hat Yai (Thailand). Faculty of Natural Resource)
Effective conditions for chitosan production from carapace and shell of black tiger shrimp (Penaeus monodon) have been developed. The optimal deproteinization of both carapace and shell involved treatment with 1.0 N NaOH at 100 deg. C for 1 h with a solids to solvent ratio of 1:6 (w/v). The optimal conditions for demineralization were 1.25 N HCl and 0.75 N HCl for deproteinized carapace and shell at ambient temperature for 0.5 h with a solids to solvent ratio of 1:12 (w/v). The chitin from carapace and shell were satisfactorily deacetylated twice with 50 NaOH (w/v) under vacuum for 30 min each time with a solids to solvent ratio of 1:15 (w/v). Chitosan products from both carapace and shell showed moderate deacetylation with 57.0 and 45.6 degree of N-acetylation, respectively and contained 7.9-8.1 crude nitrogen with remarkably low ash content
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