Identification of an Ukrainian virus isolate from Helianthus annuus L. with yellow spot mosaic symptoms
1994
Zakusilo, A. (Kiev Univ. (Ukraine). Inst. of Microbiology and Virology. Dept. of Virology) | Knyaseva, N. | Didenko, L. | Boyko, A.
An isometric virus was isolated from Helianthus annuus L. plants showing a yellow leaf spot mosaic on affected leaves. Infected plants were found in different ecological regions of Ukraine. A procedure of virus purification is described. The diametres of the virus particles were nonuniform and ranged from 50 to 120 nm. The sedimentation coefficient of the virus was 518-540 S and the floating density in the CsCl gradient was 1.22 g/cubic centimetre. The MW of proteins separated by electrophoresis amounted to 78(+-)0.9, 58(+-)0.8, 52(+-)0.2, and 27(+-)0.8 kDa, respectively. The virus was assigned to the tospoviruses for which sunflower is a new previously undescribed natural host plant
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