The development of Agrobacterium gene transfer system for apple and pear cultivars. Final Report
1994
Maheswaran, G. (Victorian Dept. of Agriculture (Australia). Inst. for Horticultural development)
A biotechnological approach has been developed to complement conventional breeding with the specific objective of introducing genes for insect pest control. This approach requires techniques for regeneration and effective genetic transformation. 2 apple cultivars and one pear have been successfully regenerated in tissue culture. The system was based on formation of direct adventitious shoots on leaf explants. Important factors in success were: an initial 4-week dark period; use of cytokinin TDZ rather than the commonly used BAP; and cultivar selection. Transgenic plants containing the gus and npt II genes were produced using Agrobacterium tumefaciens. Important factors were: the strain of bacterium; the binary vector; and an initial dark period of culture. Apples and pears have been genetically engineered with proteinase inhibitor gene which has the potential to control light brown apple moth and codling moth
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