Intoxication by heavy metals in relation to the activity of glucose-6-phosphate dehydrogenase of erythrocytes in sheep
1995
Kostova, D. | Michnova, E. | Legath, J. (Univerzita Veterinarskeho Lekarstva, Kosice (Slovakia)) | Krupicer, I.
G-6-PD activity was evaluated in the erythrocytes of sheep kept in the region contaminated by heavy metals with mercury dominating among them, and in the same animals after administration of a feed mixture containing Hg, Pb, Cd, Zn, Cr, Cu, Fe and As. Boehringer Mannheim test was used to determine the G-6-PD activity. There was no significant difference in the enzyme activity in the sheep from a contaminated region and in the animals outside the air-pollution region (control animals) before the applications of heavy metals started. The average value of G-6-PD acitivity was 13.96+/-0.94 mU.10-9 Ec in control animals and 14.39+/-1.49 mU.10-9 Ec in the animals from a contaminated region. After eight-day applications of heavy metals the G-6-PD activity increased statistically significantly to 18.71+/-2.45 mU.10-9 Ec; P 0.01, and to 23.55+/-1.87 mU.10-9 Ec after 16 days of application; P0.001. An increase in G-6-PD activity after heavy metal applications is probably a compensation mechanism in the system of erythrocyte antioxidative protection due to higher peroxidation. The long term increased intake of heavy metals from polluted air did not lead to any rise of G-6-PD activity probably due to the lower dose of heavy metals and/or due to adaptation of animal organisms to long run emission exposure. The results demonstrate that G-6-PD can be one of the biochemical indicators at organism load by heavy metals with mercury dominating among them.
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