The effect of extracts from Curcuma longa on reproduction of tobacco mosaic virus in leaves and mesophyll protoplasts of tobacco
1996
Sindelarova, M. | Burketova, L. | Sindelar, L. (Akademie Ved, Prague (Czech Republic). Ustav Experimentalni Botaniky) | Lojkova, M. | Taborsky, V.
The effect of water and acetone extract from curcuma (Curcuma longa) was investigated with respect to multiplication of tobacco mosaic virus (TMV) in leaves and mesophyll protoplasts of tobacco infected in vitro. Water extract decreased the number of local necrotic lesions on infected leaves of Nicotiana glutinosa to 68 % of the values determined in untreated controls. Water and acetone extract at a resultant dilution 1:10 inactivated TMV and PVX purificates to 74-88 % of the control. Acetone extract still inactivated TMV purificate at a dilution 1:50 (81 % of the control). Acetone extract after 72 hour cultivation did not influence the number of inoculated and noninoculated mesophyll protoplasts in any of the concentrations used, but it reduced TMV content in in vitro inoculated protoplasts.
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