Molecular marker technology to study the role of abscisic acid
1995
Quarrie, S.A. | Steed, A. | Semikhodsky, A. (John Innes Centre, Norwich (United Kingdom)) | Pekic, S. (Belgrade Univ. (Yugoslavia). Agriculture Fac.) | Lazic-Jancic, V. (Maize Research Inst., Belgrade-Zemun (Yugoslavia)) | Calestani, C. (Bologna Univ. (Italy). Agronomy Dept.) | Tuberosa, R. (Parma Univ. (Italy). Biology Dept.)
With the development of molecular marker technologies to construct detailed molecular maps of crop species it has become possible to study much more precisely than before the possible roles of ABA by analysing the distribution of quantitative trait lici (QTL). This has been done with mapping populations of maize and wheat to study the importance of endogenous ABA in determining stomatal function, water-use efficiency and root growth in plants growing under water-stressed conditions. An F2 population of maize was mapped with 100 RFLP markers and F2 plants and their F3 progenies sampled for leaf and xylem ABA contents and stomatal conductance. Correlations using genotype mean data at QTL significant for ABA content showed that stomatal conductance was more likely to be regulated by xylem ABA content than leaf ABA content. The same maize F3 population was used to identify QTL for root growth was positively associated with root ABA content. Water-use efficiency, measured as 13C discrimination, was measured in a mapping population of doubled haploid lines of wheat. Leaf ABA concentrations were also measured. Comparison of QTL for the two traits showed that leaf ABA concentrations were unlikely to have had an effect on plant water-use efficiency in those field conditions. QTL analysis is a very powerful technique for testing the likely roles of endogenous ABA.
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