Characterization of peroxisome-deficient mutants of Hansenula polymorpha
1995
Tan, X. | Titorenko, V.I. | Klei, I.J. van der | Sulter, G.J. | Haima, P. | Waterham, H.R. | Evers, M. | Harder, W. | Veenhuis, M. | Cregg, J.M. (Oregon Graduate Inst. of Science and Technology, Portland (USA). Dept. of Chemistry Biochemistry and Molecular Biology)
In the methylotrophic yeast Hansenula polymorpha, approximately 25% of all methanol-utilization-defective (Mut(-)) mutants are affected in genes required for peroxisome biogenesis (PER genes). Previously, it was reported that one group of per mutants, termed Pim-, are characterized by the presence of a few small peroxisomes with the bulk of peroxisomal enzymes located in the cytosol. Here, a second major group of per mutants that were observed to be devoid of any peroxisome-like structure (Per(-)) is described. In each Per(-) mutant, the peroxisomal methanol-pathway enzymes alcohol oxidase, catalase and dihydroxyacetone synthase were present and active but located in the cytosol. Together, the Pim(-) and Per(-) mutant collections involved mutations in 14 different PER genes. Two of the genes, PER5 and PER7, were represented by both dominant-negative and recessive alleles. Diploids resulting from crosses of dominant per strains and wild-type H. polymorpha were Mut(-) and harbored peroxisomes with abnormal morphology. This is the first report of dominant-negative mutations affecting peroxisome biogenesis.
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