[Manannase production from several mannanolytic molds on the coconut meal substrate]

anglais. Manannase is produced by mannanolytic molds on the substrate containing high mannan. The enzyme breaks down mannan and galactomannan into mannosa and galactose. Nine mesophylic molds were isolated from oil palm and king palm seeds in the earlier experiment. They could produce mannanase in the substrate of gum locust bean. Coconut meal contains of 60 percent carbohydrate, which consists of 61 percent galactomannan 26 percent mannan and 13 percent cellulose. Therefore the production of mannanase on the substrate of coconut meal (1 percent) was studied in this research. Seven isolates: Verticillium sp 1, Penicillium variable, P. lanasoviride, P. rosoe-purpureum, Penicillium sp 3, Penicillium sp 5 and Eupenicillium javanicum were chosen for determination of their enzyme activities. The production of the enzyme was compared with the one produced by: Aspergillus niger BPT and A. niger NRRL 337. The results show that E. javanicum produces the highest mannanase activity and specific activity (95,71 U/ml and 493,2 U/mg protein), while the highest protein content is produced by P. roseopurpureum (293.3 micron/ml). The saccharification activity towards coconut meal was also determined to find out the ability of mannanase to hydrolyse mannan from coconut meal. The best incubation time of saccharification activity was determined from one to twenty-four hours. The result shows that the highest saccharification activity comes from one hour incubation and is resulted from P. roseo-purpureum (5.07 U/ml), while the highest specific incubation activity is resulted from Penicillium sp3 (28.53 U/mg protein)

indonésien. Enzim mannanase dapat diproduksi oleh kapang mananolitik pada substrat kaya manan. Enzim tersebut dapat memecah senyawa manan dan galaktomanan menjadi manosa dan galaktosa. Penelitian sebelumnya telah mengisolasi sembilan kapang mesofilik dari biji palem raja dan biji sawit. Isolat tersebut dapat memproduksi mannase pada "gum locust bean". Bungkil kelapa mengandung 60 percent karbohidrat yang terdiri 61 persen galaktomanan, 26 persen mannan dan 13 persen selulosa. Karena itu pada percobaan ini dipelajari produksi enzim pada bungkil kelapa (1 persen). Tujuh jenis isolat dipilih untuk ditentukan aktivitasnya yaitu : Verticillium sp 1, Penicillium variable, P. lanasoviride, P. roseo-purpureum, Penicillium sp 3, sp 5 dan Aupenicillium javanicum. Produksi enzim dibandingkan dengan Aspergillus niger BPT dan NRRL 337. Hasil menunjukkan bahwa E. javanicum memproduksi aktivitas mananase dan spesifik yang paling tinggi (95,71 U/ml dan 493,2 U/mg protein), sedangkan kadar protein tertinggi diproduksi dari P. roseo-purpureum (293,3 micron/ml). Untuk melihat kemampuan mananase dalam memecah manan yang terkandung dalam bungkil kelapa, ditentukan pula aktifitas sakarifikasi terhadap bungkil kelapa. Penentuan masa inkubasi untuk analisis sakarifikasi antara 1 sampai 24 jam, menunjukkan bahwa kecepatan aktivitas sakarifikasi tertinggi pada masa inkubasi 1 jam. Hasil tertinggi aktivitas sakarifikasi pada masa inkubasi tersebut dihasilkan oleh P. roseo-purpureum (5,07 U/ml), sedangkan aktivitas spesifik sakarifikasi tertinggi dihasilkan enzim Penicillium sp. 3 (28,53 U/mg protein)