The ultrastructure of pothos latent virus infections [Nicotiana]
1997
Russo, M. | Rubino, L. | Martelli, G.P. (Bari Univ. (Italy). Dipartimento di Protezione delle Piante dalle Malattie)
The cytopathology of infections by the original isolate of pothos latent virus (PoLV-WT), an unclassified tombus-like virus, an infectious in vitro transcript of its RNA (PoLV-IF), and two RNA transcripts coding for non-eucapsidation-competent coat proteins (PoLV-BstB and PoLV-Nhe) was comparatively studied in Nicotiana benthamiana. In the early stages of infections, all inocula induced the same ultrastructural alterations, consisting primarily of limited peripheral vesiculation of the nuclear envelope. This led to the formation of membranous vesicles with fibrillar content that accumulated in the perinuclear region and increased in number and size as infection progressed. In older infections the cytoplasm showed many membranous bodies lined with peripheral vesicles, some of which contained droplets of electron dense material resembling lipids. Virus particles were plentiful in PoLV-WT and PoLV-IF infections, both in the cytoplasm and mitochondria. Cells infected by the two CP mutants contained granules of electron opaque material, possibly accumulations of coat protein. The cytopathology of PoLV infections differs widely from that of tombusviruses, adding strength to the notion that PoLV represents a new taxon in the family Tombusviridae
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