Comparison of Trichinella spiralis larva antigens for the detection of specific antibodies in pigs
1999
Reiterova, K. | Dubinsky, P. | Tomasovicova, O. | Dvoroznakova, E. (Slovenska Akademia Vied, Kosice (Slovak Republic). Parazitologicky Ustav) | Klimenko, V.V.
An immunoenzymatic method was used to compare Trichinella spiralis muscle larva excretory-secretory antigen (TES), somatic antigen (TE) and its fraction (TF) obtained by gel filtration. The sensitivity and specificity of the assay was verified on sera of pigs experimentally infected with T. spiralis, sera of healthy pigs and sera of pigs experimentally infected with Ascaris suum and Oesophagostomum spp. Extinction values recorded throughout the experiment for TE and TF antigens were higher than those for TES antigen. The presence of anti-Trichinella antibodies was proved by TE and TF antigens as early as day 21 while TES antigen only on day 28 post infection. Using TF and TES antigens, a 100 % specificity was achieved, however, when TE antigen was used cross reactions in A. suum infection were observed. For the diagnosis of trichinellosis in pigs, the ELISA is recommended with using somatic larval antigen fraction of T. spiralis (TF).
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