Comparative study of hydrolysis of various starches by alpha-amylase and glucoamylase in PEG-dextran and PEG-substrate aqueous two phase systems
1998
Karakatsanis, A. | Liakopoulou-Kyriakides, M.
Various crude starches were hydrolyzed by the synergistic action of alpha-amylase and glucoamylase in PEG (polyethylene glycol)-dextran and PEG-substrate aqueous two phase systems. The hydrolysis products were determined, at different temperatures, by the chromatometric method of phenol-sulfurific acid and by HPLC. It was found that even the use of aqueous biphasic systems presents in general advantages in terms of separation of the products in one phase. PEG-substrate system can be used successfully in the cases of corn and rice starches. The main advantage in the use of starch as one of the polymer needed for the formation of two phases, is that the substitution of the dextran polymer decreases remarkably the cost ofthe reaction. Prior gelatinization of the starch used, gives higher yields of glucose than in the case of non gelatinized starch and the separation of the phases is satisfactory.
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