Analysis of factors affecting embryogenesis in microspore cultures of some cruciferous vegetables
1998
Vyvadilova, M. | Klima, M. | Kucera, V. (Vyzkumny Ustav Rostlinne Vyroby, Prague-Ruzyne (Czech Republic))
Five factors affecting embryogenic processes in microspore culture of Brassica oleracea were tested: donor plant genotype, donor plant growing conditions (growth chamber, glasshouse, field), developmental stage of isolated microspore population, level of the elevated temperature (30; 32,5 and 35 deg C) and the effect of culture medium. Two F1 hybrid cultivars of broccoli, four hybrids of Brussels sprouts, four genotypes of kohlrabi, sixteen cultivars of cauliflower, and three genotypes of cabbage were used for experiments. The production of developed embryos per Petri dish was chosen as a source of data for statistical analysis. Regeneration of whole plants was also evaluated. Besides these characteristics the course of embryogenesis in individual cultivars and breeding materials was observed and evaluated. The results of statistical analysis did not prove a significant effect of any tested level of individual factors. Microspore division was detected in all tested botanical varieties. Production of developed embryos and subsequently of the whole plants was achieved in one cultivar of broccoli, one genotype of kohlrabi and six cultivars of cauliflower.
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