The enzymatic determination of starch in food, feed and raw materials in the starch industry
1998
Brunt, K. | Sanders, P. | Rozema, T.
An enzymatic starch determination which can be used for the analysis of starch in a very broad range of different samples is evaluated, ranging from starch in plants, feed and food to industrial applications as starch in starch. The method is based on a complete enzymatic conversion of the starch into glucose, followed by an enzymatic determination of the glucose. A statistical evaluation of the method based on the analysis of 15 different matrices is presented. The performance of the method is compared with other methods for the determination of starch. Especially the sample desintegration and solubilization in this method perform better than in other methods which appear to be applicable in just a limited number of different sample matrices. The protocol described in this paper has been tested in an interlaboratory study, involving seven laboratories and four samples. The determination can be used in routine for the analysis of about 50 samples simultaneously, it has a good repeatability, a high selectivity, and is good applicable to samples from agricultural and industrial origin.
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