Micropropagation of Cupressus sempervirens L. and Chamaecyparis lawsoniana (A. MURR.) PAR.
1997
Spanos, K.A. | Pirrie, A. | Woodward, S.
Shoots from 18-month-old seedlings of Cupressus sempervirens and Chamaecyparis lawsoniana were established in vitro on modified MURASHIGE and SKOOG medium. Proliferation of axillary shoots occurred without addition of benzyladenine, although a significant increase in numbers of shoots resulted on addition of 0.001 mg.l(-1) to 1.0 mg.l(-1) benzyladenine. Following conditioning on a growth regulator-free medium for 28 days, 95% of C. sempervirens shoots rooted on 1/2 strength medium containing 1% sucrose and 0.5 mg.l(-1) indole butyric acid. Similar levels of rooting were recorded with Chamaecyparis lawsoniana on the same medium containing 1 mg.l(-1) indole butyric acid. C. sempervirens shoots were also rooted in non-sterile conditions. Over 80% of rooted shoots of C. sempervirens were weaned into glasshouse conditions. The significance of these results in relation to propagation of cypress clones resistant to Seiridium canker is discussed.
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