Detection of DNA polymorphism in potato cultivars using RAPD technique
2000
Polzerova, H. | Ptacek, J. (Vyzkumny Ustav Bramborarsky, Havlickuv Brod (Czech Republic))
The RAPD procedure was used to study genetic diversity of 30 potato (Solanum tuberosum) genotypes, representing mainly Czech potato cultivars. Genomic DNA was isolated by five methods and these techniques were compared. These DNA were used as template in RAPD amplification and no variations in the banding patterns were found between reactions made from various DNA extraction. Some variations between RAPD products were observed when different DNA polymerases were used. Amplification with six decamer primers generated 66 DNA fragments, ranging in size from 178 bp to 1847 bp, of which 46 were polymorphic products. The similarities of RAPD profiles were estimated by the Jaccard`s coefficient and then the data were processed by cluster analysis (UPGMA). Each genotype was identified and distinguished from the others. Our results indicate that RAPD technology is a rapid technique usable for identification of potato genotypes.
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